Small Nuclear RNA 64 (snoRNA64): A novel Tumor Biomarker for Pancreatic Cancer

The pancreatic ductal adenocarcinoma (PDAC), which represents over 90% of cancer cases, has the highest proliferative and metastatic rate in comparison to other compartments. This study is designed determine whether small nucleolar RNA, H/ACA box 64 (snoRNA64) associated with initiation progression. Gene expression data from Expression Omnibus (GEO) repository have shown that snoRNA64 reduced primary as compared normal tissues based on statistical analysis Silico analysis. Using qPCR techniques, cell lines include PK-1, PK-8, PK-4, Mia PaCa-2 different levels snoRNA64, including PaCa-2. level correlated line epithelial or mesenchymal characteristics. Cell displaying characteristics such PK-8 show high meanwhile, low snoRNA64. After knocking down expression, markers E. cadherin (E-cad) Cytokeratin-8 (CK-8) are decreased, while Vimentin (Vim), Cytokeratin-19 (CK-19), Metalloprotease -2 (MMP-2), Metalloprotease-3 (MMP-3) activated. Those changes suggest responding knock protocol increase function. Together, may participate transition (EMT) (MET), during metastasis these processes crucial. In addition, be considered a potential diagnostic biomarker for both early invasive stages PDAC. And due its gradual decreases, it barrier tumor